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I0259
Sigma
Insulin human
powder, recombinant, expressed in Escherichia coli, cell culture tested
| CAS Number: | 11061-68-0 |
| Linear Formula: | C257H383N65O77S6 |
| Molecular Weight: | 5807.57 |
| EC Number: | 234-279-7 |
| MDL number: | MFCD00131380 |
Description
| Biochem/physiol Actions | Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat. |
Properties
| recombinant | expressed in Escherichia coli |
| form | powder |
| potency | ≥27.5 USP units per mg (dry basis) |
| solubility | 10 mM HCl: 20 mg/mL |
| suitability | cell culture tested |
| Gene Information | human ... INS(3630) |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | NWG |
| RTECS | NM8900250 |
References
| reference | Smith, L.F., Species variation in the amino acid sequence of insulin. Am. J. Med. 40, 662-666, (1966) |
| Kono, T., Insulin-sensitive glucose transport. Vitam. Horm. 7, 103-154, (1988) | |
| Morihara, K., et al., Semi-synthesis of human insulin by trypsin-catalysed replacement of Ala-B30 by Thr in porcine insulin. Nature 280, 412-413, (1979) |
